Journal: Journal of Visualized Experiments : JoVE

Article Title: Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

doi: 10.3791/3791

Figure Lengend Snippet:

Article Snippet: L17 , Biotinylated Solanum Tuberosum (Potato) Lectin , STL , Vector Laboratories , BK-3000.

Techniques: Plasmid Preparation, Positive Control

Journal: American Journal of Translational Research

Article Title: Glycosylation patterns and PHA-E-associated glycoprotein profiling associated with early hepatic encephalopathy in Chinese hepatocellular carcinoma patients

doi:

Figure Lengend Snippet: Different glycosylation patterns in sera from Chinese HCC patients with and without early HE. A. Specific carbohydrate epitopes and quantitative results of lectins had statistical significance between sera form Chinese HCC patients with and without early HE. Data was the average ± SD, *P<0.05, **P<0.01, ***P<0.001. B. Validation of different glycosylation patterns by lectin blotting. Coomassie brilliant blue staining was used to show the protein loading. Signal strength of JAC was increased, while signal strengths of PHA-E, RCA-I, STL, WFL and WGA were all decreased in sera from Chinese HCC patients with early HE compared with in sera from Chinese HCC patients without early HE.

Article Snippet: After blocking, the membranes were incubated with biotinylated jacalin (JAC), phaseolus vulgaris erythroagglutinin (PHA-E), ricinus communis agglutinin I (RCA-I), solanum tuberosum lectin (STL), wisteria floribunda lectin (WFL) and wheat germ agglutinin (WGA, Vector Laboratories, Burlingame, CA, USA), respectively.

Techniques: Staining

Journal: PLoS ONE

Article Title: Delayed histochemical alterations within the neurovascular unit due to transient focal cerebral ischemia and experimental treatment with neurotrophic factors

doi: 10.1371/journal.pone.0174996

Figure Lengend Snippet: Triple fluorescence labeling.

Article Snippet: Subsequently, the sections were incubated overnight with a mixture of rabbit-anti-collagen IV (Merck Millipore, Billerica, MA; 1:200 in the blocking solution) and biotinylated STL (Vector Laboratories, Burlingame, CA).

Techniques: Fluorescence, Labeling, Marker, Plasmid Preparation

Journal: PLoS ONE

Article Title: Delayed histochemical alterations within the neurovascular unit due to transient focal cerebral ischemia and experimental treatment with neurotrophic factors

doi: 10.1371/journal.pone.0174996

Figure Lengend Snippet: Lectin-histochemical staining with biotinylated Solanum tuberosum lectin (STL) and Cy2-streptavidin (A) revealed a persisting, but thinned endothelium in stroke-affected neocortical areas. The concomitant Cy3-immunolabeling based on rabbit-anti-serum albumin (Alb, A’) showed the BBB permeability marker within the parenchyma which became even clearer by the merged picture (A”). The overlay of STL and albumin clearly revealed stroke-induced leakage of the BBB resulting in intra-parenchymal albumin-immunoreactivity also visible in several perikarya, whereas another animal displayed less albumin-immunopositive somata in an infarcted striatal border region (B). Scale bar in A” (also valid for A and A’) = 75 μm, in B = 100 μm.

Article Snippet: Subsequently, the sections were incubated overnight with a mixture of rabbit-anti-collagen IV (Merck Millipore, Billerica, MA; 1:200 in the blocking solution) and biotinylated STL (Vector Laboratories, Burlingame, CA).

Techniques: Staining, Immunolabeling, Permeability, Marker

Journal: Cell

Article Title: Membrane Microdomain Disassembly Inhibits MRSA Antibiotic Resistance

doi: 10.1016/j.cell.2017.10.012

Figure Lengend Snippet: Identification of FMM-Constituent Lipids (A) Ion chromatogram of FMM lipid markers in DRM (left) and DSM (right) fractions, labeled with RT and m/z ratios. Lipid abundance represented in absorbance units (B) Fragmentation pattern of FMM lipid markers at negative (top) and positive (bottom) ESI by product ion scan (MS/MS). Common fragments with respective MW and tentative formulas are shown. (C) (Top) TLC detection of staphyloxanthin lipids in DRM and DSM fractions of WT and Δ crt mutant. Staphyloxanthin lipids are visualized as yellow-pigmented bands (arrowheads). (D) UV-visible spectroscopy of purified staphyloxanthin and DRM and DSM samples (WT and Δ crt mutant). Arrowheads indicate characteristic 463- and 490-nm staphyloxanthin peaks. (E) Fluorescein-labeled lectin binding assay to WT and Δ crt DRM samples. WGA, wheat germ agglutinin; STL, Solanum tuberosum lectin; RCA, Ricinus communis agglutinin; DBA, Dolichos biflorus agglutinin; UEA, Ulex europaeus agglutinin; ConA, concanavalin A. (F) Relative abundance of FMM lipid markers in WT and Δ crt mutant using ion chromatography. Data shown as mean ± SD for three biological replicates (n = 3). (G) Tentative molecular structure and fragmentation pattern (blue, negative ESI; red, positive ESI) of staphyloxanthin-related FMM lipid markers. See also and and and .

Article Snippet: Fluorescein Solanum tuberosum (potato) Lectin , Vector Laboratories , Cat#FL-1161.

Techniques: Labeling, Tandem Mass Spectroscopy, Mutagenesis, Spectroscopy, Purification, Binding Assay, Ion Chromatography

Journal: Cell

Article Title: Membrane Microdomain Disassembly Inhibits MRSA Antibiotic Resistance

doi: 10.1016/j.cell.2017.10.012

Figure Lengend Snippet:

Article Snippet: Fluorescein Solanum tuberosum (potato) Lectin , Vector Laboratories , Cat#FL-1161.

Techniques: Recombinant, Plasmid Preparation, Electron Microscopy, Protease Inhibitor, Software, Western Blot, Microscopy, Mass Spectrometry, Size-exclusion Chromatography, Fluorescence, Tomography

Journal: Brain Pathology

Article Title: Common Impact of Chronic Kidney Disease and Brain Microhemorrhages on Cerebral Aβ Pathology in SHRSP

doi: 10.1111/bpa.12384

Figure Lengend Snippet: Aβ CNS deposition, microhemorrhages and tubulointerstitial renal damage in SHRSP. Dense parenchymal (A–A3 & C1), pericapillary (B, C (arrows) & C2–C4) Aβ deposits, small perivascular bleeds (D, E & F (arrows) & D1), infarcts (arrowheads in G) and IgG leakage throughout the small vessel walls (K–M) are part of the age‐dependent mixed cerebral pathologies in SHRSP. Iron and Aβ depositions are colocalized in the rat brain (A1–A3 & C1–C4). In the brains of these animals, recent microhemorrhages co‐occur with small ischemic lesions (arrowheads in E & F), thromboses (* in F) and with liquefaction ischemic lesions containing haem‐laden macrophages (arrowheads in G). Peritubular capillary erythrocyte accumulations (H, arrow), tubular protein cylinders (I, arrow) and hyperplastic arteriolosclerosis (J, arrows) indicate combined tubulointerstitial and vascular/hypertensive kidney damage in SHRSP. B, K, L, M – IHC, STL ‐ solanum tuberosum lectin‐fluorescein isothiocyanate (endothelial marker), zymo – in situ zymography showing matrix metalloproteinases 2,9 activity visualized with the use of gelatin conjugated with FITC (DQ gelatin, Invitrogen), IgG – immunoglobulin G, representing the IgG leakage, visualized with the use of an anti‐rat IgG secondary antibody Cy3 conjugated (Dianova, Hamburg, Germany, 1:500), DAPI ‐ 4′.6‐diamidino‐2‐phenylindole (nuclear staining), A–A3 – Prussian blue/CR staining, C, C2–C4 – Prussian blue/Thioflavine S staining, C1 ‐ Prussian blue/Thioflavine T staining, D–J – HE staining. A & A1 – corpus callosum, A2, A3, C1, C2 & D–G – cortex, B, C, C 3 & C4 – hippocampus, D1 ‐ basal ganglia, H & I – kidney medulla, J – kidney cortex. A1, B1, C3, C4 – magnifications of the respective subfigures.

Article Snippet: Five brain sections per animal adjacent to those with suspected CR‐positive Aβ deposits were stained with STL‐FITC (solanum tuberosum lectin‐fluorescein isothiocyanate, Axxora, Enzo Life Sciences GmBH, Loerrach, Germany, 1:500, endothelial marker), anti‐rodent Aβ (Covance, Dedham, MA, USA, 1:500, specific for rodent Aβ), and DAPI (4′.6‐diamidino‐2‐phenylindole, MoBiTec GmbH, Germany, 1:10 000, nuclear staining).

Techniques: Marker, In Situ, Zymography, Activity Assay, Staining